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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of beta-hemolytic streptococci.
Zhou, Chunmei; Tao, Lili; Hu, Bijie; Ma, Jian; Ye, Xiangru; Huang, Shenglei; Ma, Yan; Shan, Yuzhang.
Afiliação
  • Zhou C; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
  • Tao L; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
  • Hu B; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
  • Ma J; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
  • Ye X; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
  • Huang S; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
  • Ma Y; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
  • Shan Y; 1 Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2 Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China ; 3 Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 2
J Thorac Dis ; 7(4): 591-5, 2015 Apr.
Article em En | MEDLINE | ID: mdl-25973224
ABSTRACT

OBJECTIVE:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as promising technology for species identification. The purpose of this investigation was to compare the performance of MS and the traditional method for identification of beta-hemolytic streptococci (BHS).

METHODS:

Clinical BHS isolates were identified by the BD Phoenix SMIC/ID Streptococcal panels, and two MALDI-TOF MS platforms the VITEK MS and the Bruker MALDI Biotyper systems respectively. In case of discordant results, 16sRNA sequencing was performed to provide the reference ID.

RESULTS:

A total of 96 isolates of BHS were analyzed. Thirty-six isolates (20.8%) were re-tested by BD Phoenix for identification failure; and four isolates (4.2%) were rerun on the Bruker system for low identification score. No isolate need a second run for identification by Vitek MS system. Overall, BD Phoenix, BioTyper and Vitek MS automated system accurately identified 76 strains (79.2%), 91 (94.7%) strains and 92 (95.8%) strains, respectively.

CONCLUSIONS:

Our study suggests that MALDI-TOF MS is a superior method to conventional phenotypic methods for BHS identification.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Thorac Dis Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Thorac Dis Ano de publicação: 2015 Tipo de documento: Article