Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution.
Biochem Soc Trans
; 43(3): 309-14, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-26009168
ABSTRACT
There is a limited range of methods available to characterize macromolecular organization in cells on length scales from 5-50 nm. We review methods currently available and show the latest results from a new single-molecule localization-based method, fluorophore localization imaging with photobleaching (FLImP), using the epidermal growth factor (EGF) receptor (EGFR) as an example system. Our measurements show that FLImP is capable of achieving spatial resolution in the order of 6 nm.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Substâncias Macromoleculares
/
Fator de Crescimento Epidérmico
/
Receptores ErbB
Limite:
Humans
Idioma:
En
Revista:
Biochem Soc Trans
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Reino Unido