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Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution.
Needham, Sarah R; Zanetti-Domingues, Laura C; Scherer, Kathrin M; Hirsch, Michael; Rolfe, Daniel J; Roberts, Selene K; Martin-Fernandez, Marisa L; Clarke, David T; Tynan, Christopher J.
Afiliação
  • Needham SR; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Zanetti-Domingues LC; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Scherer KM; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Hirsch M; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Rolfe DJ; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Roberts SK; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Martin-Fernandez ML; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Clarke DT; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
  • Tynan CJ; *Central Laser Facility, Research Complex at Harwell, STFC Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0FA, U.K.
Biochem Soc Trans ; 43(3): 309-14, 2015 Jun.
Article em En | MEDLINE | ID: mdl-26009168
ABSTRACT
There is a limited range of methods available to characterize macromolecular organization in cells on length scales from 5-50 nm. We review methods currently available and show the latest results from a new single-molecule localization-based method, fluorophore localization imaging with photobleaching (FLImP), using the epidermal growth factor (EGF) receptor (EGFR) as an example system. Our measurements show that FLImP is capable of achieving spatial resolution in the order of 6 nm.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Substâncias Macromoleculares / Fator de Crescimento Epidérmico / Receptores ErbB Limite: Humans Idioma: En Revista: Biochem Soc Trans Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Reino Unido
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Substâncias Macromoleculares / Fator de Crescimento Epidérmico / Receptores ErbB Limite: Humans Idioma: En Revista: Biochem Soc Trans Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Reino Unido