Chromosome Conformation Capture Carbon Copy (5C) in Budding Yeast.
Cold Spring Harb Protoc
; 2015(6): 593-8, 2015 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-26034306
ABSTRACT
Chromosome conformation capture carbon copy (5C) is a high-throughput method for detecting ligation products of interest in a chromosome conformation capture (3C) library. 5C uses ligation-mediated amplification (LMA) to generate carbon copies of 3C ligation product junctions using single-stranded oligonucleotide probes. This procedure produces a 5C library of short DNA molecules which represent the interactions between the corresponding restriction fragments. The 5C library can be amplified using universal primers containing the Illumina paired-end adaptor sequences for subsequent high-throughput sequencing.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromossomos
/
Saccharomycetales
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2015
Tipo de documento:
Article