Towards time-resolved serial crystallography in a microfluidic device.
Acta Crystallogr F Struct Biol Commun
; 71(Pt 7): 823-30, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-26144226
ABSTRACT
Serial methods for crystallography have the potential to enable dynamic structural studies of protein targets that have been resistant to single-crystal strategies. The use of serial data-collection strategies can circumvent challenges associated with radiation damage and repeated reaction initiation. This work utilizes a microfluidic crystallization platform for the serial time-resolved Laue diffraction analysis of macroscopic crystals of photoactive yellow protein (PYP). Reaction initiation was achieved via pulsed laser illumination, and the resultant electron-density difference maps clearly depict the expected pR(1)/pR(E46Q) and pR(2)/pR(CW) states at 10â
µs and the pB1 intermediate at 1â
ms. The strategies presented here have tremendous potential for extension to chemical triggering methods for reaction initiation and for extension to dynamic, multivariable analyses.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Difração de Raios X
/
Fotorreceptores Microbianos
/
Dispositivos Lab-On-A-Chip
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos