ß-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells.
Biomed Res Int
; 2015: 153987, 2015.
Article
em En
| MEDLINE
| ID: mdl-26221582
ABSTRACT
OBJECTIVE:
To investigate the effects of ß-Elemene (ß-ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase IIα (TOPO IIα) expression and activity of human hepatocarcinoma HepG-2 cells.METHODS:
After treatment with ß-ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope. Cell proliferation was assessed using an MTT assay, cell cycles were analyzed using flow cytometry, and apoptosis was detected by Annexin V/PI staining. The expression of TOPO I and TOPO IIα was analyzed by Western blot techniques, and their activity was measured using the TOPO I-mediated, supercoiled pBR322 DNA relaxation and TOPO IIα-mediated Kinetoplast DNA (kDNA) decatenation assays, respectively. Supercoiled pBR322 and kDNA were also used to determine the direct effect of ß-ELE on DNA breaks.RESULTS:
ß-ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner. ß-ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO IIα in a dose-dependent manner. ß-ELE also inhibited TOPO I- and TOPO IIα-mediated DNA relaxation but did not directly induce DNA breakage at any concentration.CONCLUSION:
ß-ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO IIα.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sesquiterpenos
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DNA Topoisomerases Tipo II
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DNA Topoisomerases Tipo I
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Carcinoma Hepatocelular
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Proteínas de Ligação a DNA
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Neoplasias Hepáticas
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Antígenos de Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Biomed Res Int
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China