Paclitaxel Inhibits Expression of Neuronal Nitric Oxide Synthase and Prevents Mitochondrial Dysfunction in Spinal Ventral Horn in Rats After C7 Spinal Root Avulsion.
Turk Neurosurg
; 25(4): 617-24, 2015.
Article
em En
| MEDLINE
| ID: mdl-26242340
ABSTRACT
AIM:
This study evaluated the neuroprotective effect of intrathecally infused paclitaxel in the prevention of motoneuron death and mitochondrial dysfunction following brachial plexus avulsion injury. MATERIAL ANDMETHODS:
Brachial root avulsion injury was induced in Sprague-Dawley rats. The Paclitaxel treatment group (n = 32) received a 5-d intrathecal infusion of paclitaxel (256 ng/d) via a micro infusion pump, whereas the Control group (n = 32) received normal saline. The cervical cord was harvested at survival times of 1, 2, 4, and 6 wk (n = 8 each). The number of surviving and nNOS-positive motoneurons at the injury level in the ventral horn was determined with NADPH-d histochemistry. Mitochondrial function at this location was measured with CcO histochemistry and densitometry. An independent t-test was applied to detect differences between the study groups at specific survival times.RESULTS:
The Paclitaxel treatment group showed a significant relative reduction in nNOS expression at 2, 4, and 6 wk, and significantly improved mitochondrial function at 4 and 6 wk. Motoneuron survival was significantly increased at 2, 4, and 6 wk.CONCLUSION:
Paclitaxel has a significant neuroprotective effect against spinal motoneuron degeneration following brachial plexus avulsion injury, which involves inhibition of nNOS expression and prevention of mitochondrial dysfunction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raízes Nervosas Espinhais
/
Paclitaxel
/
Fármacos Neuroprotetores
/
Doenças Mitocondriais
/
Óxido Nítrico Sintase Tipo I
/
Células do Corno Anterior
/
Antineoplásicos Fitogênicos
Limite:
Animals
Idioma:
En
Revista:
Turk Neurosurg
Assunto da revista:
NEUROCIRURGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Malásia