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Neuronal Survival, Morphology and Outgrowth of Spiral Ganglion Neurons Using a Defined Growth Factor Combination.
Schwieger, Jana; Warnecke, Athanasia; Lenarz, Thomas; Esser, Karl-Heinz; Scheper, Verena.
Afiliação
  • Schwieger J; Department of Otolaryngology, Hannover Medical School, Hannover, Germany; Institute of Zoology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
  • Warnecke A; Department of Otolaryngology, Hannover Medical School, Hannover, Germany; Cluster of Excellence Hearing4all, German Research Foundation, Hannover, Germany.
  • Lenarz T; Department of Otolaryngology, Hannover Medical School, Hannover, Germany; Cluster of Excellence Hearing4all, German Research Foundation, Hannover, Germany.
  • Esser KH; Institute of Zoology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
  • Scheper V; Department of Otolaryngology, Hannover Medical School, Hannover, Germany; Institute of Audioneurotechnology, Hannover Medical School, Hannover, Germany.
PLoS One ; 10(8): e0133680, 2015.
Article em En | MEDLINE | ID: mdl-26263175
ABSTRACT

OBJECTIVES:

The functionality of cochlear implants (CI) depends, among others, on the number and excitability of surviving spiral ganglion neurons (SGN). The spatial separation between the SGN, located in the bony axis of the inner ear, and the CI, which is inserted in the scala tympani, results in suboptimal performance of CI patients and may be decreased by attracting the SGN neurites towards the electrode contacts. Neurotrophic factors (NTFs) can support neuronal survival and neurite outgrowth.

METHODS:

Since brain-derived neurotrophic factor (BDNF) is well known for its neuroprotective effect and ciliary neurotrophic factor (CNTF) increases neurite outgrowth, we evaluated if the combination of BDNF and CNTF leads to an enhanced neuronal survival with extended neurite outgrowth. Both NTFs were added in effective high concentrations (BDNF 50 ng/ml, CNTF 100 ng/ml), alone and in combination, to cultured dissociated SGN of neonatal rats for 48 hours.

RESULTS:

The neuronal survival and neurite outgrowth were significantly higher in SGN treated with the combination of the two NTFs compared to treatment with each factor alone. Additionally, with respect to the morphology, the combination of BDNF and CNTF leads to a significantly higher number of bipolar neurons and a decreased number of neurons without neurites in culture.

CONCLUSION:

The combination of BDNF and CNTF shows a great potential to increase the neuronal survival and the number of bipolar neurons in vitro and to regenerate retracted nerve fibers.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gânglio Espiral da Cóclea / Peptídeos e Proteínas de Sinalização Intercelular / Neurônios Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gânglio Espiral da Cóclea / Peptídeos e Proteínas de Sinalização Intercelular / Neurônios Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha