Reduced blastocyst formation in reduced culture volume.
J Assist Reprod Genet
; 32(9): 1365-70, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26292800
ABSTRACT
PURPOSE:
The aim of this prospective sibling oocyte study was to evaluate whether reduced culture volume improves blastocyst formation.METHODS:
Twenty-three patients with extended embryo culture until day 5 were selected for the study. After injection, 345 sibling oocytes were individually cultured in either 25 or 7 µl droplets of Origio cleavage medium under oil. On day 3 of development, embryos were transferred to droplets with the corresponding volume of Origio blastocyst culture medium. Fertilization and embryo quality on day 3 and day 5/6 were evaluated.RESULTS:
No statistically significant difference (p = 0.326) in fertilization rate was observed (81.3 versus 83.0 %). There was no significant difference in terms of the number of excellent and good-quality embryos obtained on day 3 between both groups (p = 0.655). Embryo culture in 25 µl droplets led to more embryos with a higher cell number when compared to 7 µl culture (p = 0.024). On day 3, 132 and 131 embryos were considered for further culture until day 5/6. Blastulation rates were significantly higher in the 25 µl group (75.0 versus 61.6 %; p = 0.017) and significantly more day 5 embryos with excellent and good quality were found in this group (54.5 versus 40.5 %; p = 0.026). Finally, the utilization rates expressed per mature oocyte (41.4 versus 29.8 %; p = 0.043), per fertilized oocyte (50.7 versus 36.6 %; p = 0.023), and per day 3 embryo undergoing extended culture to day 5/6 (54.5 versus 39.7 %; p = 0.019) were all significantly higher in the 25 µl group.CONCLUSION:
Reduced culture volume (7 µl) negatively impacts early development by reducing the cell number on day 3 and both blastocyst formation and quality.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Espermatozoides
/
Blastocisto
/
Fertilização in vitro
/
Técnicas de Cultura Embrionária
/
Embrião de Mamíferos
/
Fertilização
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Adult
/
Female
/
Humans
/
Male
/
Pregnancy
Idioma:
En
Revista:
J Assist Reprod Genet
Assunto da revista:
GENETICA
/
MEDICINA REPRODUTIVA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Bélgica