Plasma carnosine, but not muscle carnosine, attenuates high-fat diet-induced metabolic stress.
Appl Physiol Nutr Metab
; 40(9): 868-76, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26307517
ABSTRACT
There is growing in vivo evidence that the dipeptide carnosine has protective effects in metabolic diseases. A critical unanswered question is whether its site of action is tissues or plasma. This was investigated using oral carnosine versus ß-alanine supplementation in a high-fat diet rat model. Thirty-six male Sprague-Dawley rats received a control diet (CON), a high-fat diet (HF; 60% of energy from fat), the HF diet with 1.8% carnosine (HFcar), or the HF diet with 1% ß-alanine (HFba), as ß-alanine can increase muscle carnosine without increasing plasma carnosine. Insulin sensitivity, inflammatory signaling, and lipoxidative stress were determined in skeletal muscle and blood. In a pilot study, urine was collected. The 3 HF groups were significantly heavier than the CON group. Muscle carnosine concentrations increased equally in the HFcar and HFba groups, while elevated plasma carnosine levels and carnosine-4-hydroxy-2-nonenal adducts were detected only in the HFcar group. Elevated plasma and urine N(ε)-(carboxymethyl)lysine in HF rats was reduced by â¼50% in the HFcar group but not in the HFba group. Likewise, inducible nitric oxide synthase mRNA was decreased by 47% (p < 0.05) in the HFcar group, but not in the HFba group, compared with HF rats. We conclude that plasma carnosine, but not muscle carnosine, is involved in preventing early-stage lipoxidation in the circulation and inflammatory signaling in the muscle of rats.
Palavras-chave
advanced glycation end products; advanced lipoxidation end products; aptitude à désaltérer; bêta-alanine; carnosine conjugates; conjugués de carnosine; inflammatory signaling; produits finaux de la glycation avancée; produits finaux de la lipoxydation avancée; quenching ability; signalisation de l'inflammation; ß-alanine
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carnosina
/
Peroxidação de Lipídeos
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Músculo Esquelético
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Suplementos Nutricionais
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Dieta Hiperlipídica
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Inflamação
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Anti-Inflamatórios
Tipo de estudo:
Etiology_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Appl Physiol Nutr Metab
Assunto da revista:
CIENCIAS DA NUTRICAO
/
FISIOLOGIA
/
MEDICINA ESPORTIVA
/
METABOLISMO
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Bélgica