BamHash: a checksum program for verifying the integrity of sequence data.
Bioinformatics
; 32(1): 140-1, 2016 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-26363028
ABSTRACT
SUMMARY:
Large resequencing projects require a significant amount of storage for raw sequences, as well as alignment files. Because the raw sequences are redundant once the alignment has been generated, it is possible to keep only the alignment files. We present BamHash, a checksum based method to ensure that the read pairs in FASTQ files match exactly the read pairs stored in BAM files, regardless of the ordering of reads. BamHash can be used to verify the integrity of the files stored and discover any discrepancies. Thus, BamHash can be used to determine if it is safe to delete the FASTQ files storing raw sequencing read after alignment, without the loss of data. AVAILABILITY AND IMPLEMENTATION The software is implemented in C++, GPL licensed and available at https//github.com/DecodeGenetics/BamHash CONTACT pmelsted@hi.is.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Estatística como Assunto
/
Análise de Sequência
Limite:
Humans
Idioma:
En
Revista:
Bioinformatics
Assunto da revista:
INFORMATICA MEDICA
Ano de publicação:
2016
Tipo de documento:
Article