Spatiotemporally Controlled Reorganization of Signaling Complexes in the Plasma Membrane of Living Cells.

Wedeking, Tim; Löchte, Sara; Birkholz, Oliver; Wallenstein, Alexander; Trahe, Julia; Klingauf, Jürgen; Piehler, Jacob; You, Changjiang

Wedeking, Tim; Löchte, Sara; Birkholz, Oliver; Wallenstein, Alexander; Trahe, Julia; Klingauf, Jürgen; Piehler, Jacob; You, Changjiang.

Afiliação

Wedeking T; Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
Löchte S; Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
Birkholz O; Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
Wallenstein A; Department of BiologyUniversity of Osnabrück, Barbarastr. 11, Osnabrück, 49076, Germany.
Trahe J; Institute of Medical Physics and Biophysics, University of Münster, Robert-Koch-Str. 31, Münster, 48149, Germany.
Klingauf J; Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), University of Münster, Münster, 48149, Germany.
Piehler J; Institute of Medical Physics and Biophysics, University of Münster, Robert-Koch-Str. 31, Münster, 48149, Germany.
You C; Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), University of Münster, Münster, 48149, Germany.

Small ; 11(44): 5912-8, 2015 Nov 25.

Article em En | MEDLINE | ID: mdl-26421417

ABSTRACT

ABSTRACT

Triggered immobilization of proteins in the plasma membrane of living cells into functional micropatterns is established by using an adaptor protein, which is comprised of an antiGFP nanobody fused to the HaloTag protein. Efficient in situ reorganization of the type I interferon receptor subunits as well as intact, fully functional signaling complexes in living cells are achieved by this method.

Assuntos

Membrana Celular/metabolismo; Transdução de Sinais; Sobrevivência Celular; Células HeLa; Humanos; Proteínas Imobilizadas/metabolismo; Proteínas de Membrana/metabolismo; Microtecnologia; Receptores de Superfície Celular/metabolismo

Palavras-chave

cell micropatterning; cytokine signaling; fluorescence imaging; imaging, plasma membranes, type I interferon receptors; microcontact printing

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução de Sinais / Membrana Celular Limite: Humans Idioma: En Revista: Small Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Alemanha

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