lin28 proteins promote expression of 17â¼92 family miRNAs during amphibian development.
Dev Dyn
; 245(1): 34-46, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26447465
ABSTRACT
BACKGROUND:
Lin28 proteins are post-transcriptional regulators of gene expression with multiple roles in development and the regulation of pluripotency in stem cells. Much attention has focussed on Lin28 proteins as negative regulators of let-7 miRNA biogenesis; a function that is conserved in several animal groups and in multiple processes. However, there is increasing evidence that Lin28 proteins have additional roles, distinct from regulation of let-7 abundance. We have previously demonstrated that lin28 proteins have functions associated with the regulation of early cell lineage specification in Xenopus embryos, independent of a lin28/let-7 regulatory axis. However, the nature of lin28 targets in Xenopus development remains obscure.RESULTS:
Here, we show that mir-17â¼92 and mir-106â¼363 cluster miRNAs are down-regulated in response to lin28 knockdown, and RNAs from these clusters are co-expressed with lin28 genes during germ layer specification. Mature miRNAs derived from pre-mir-363 are most sensitive to lin28 inhibition. We demonstrate that lin28a binds to the terminal loop of pre-mir-363 with an affinity similar to that of let-7, and that this high affinity interaction requires to conserved a GGAG motif.CONCLUSIONS:
Our data suggest a novel function for amphibian lin28 proteins as positive regulators of mir-17â¼92 family miRNAs.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
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Proteínas de Ligação a RNA
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Proteínas de Xenopus
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MicroRNAs
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Desenvolvimento Embrionário
Limite:
Animals
Idioma:
En
Revista:
Dev Dyn
Assunto da revista:
ANATOMIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Reino Unido