Beneficial effect of Citrus limon peel aqueous methanol extract on experimentally induced urolithic rats.
Pharm Biol
; 54(5): 759-69, 2016.
Article
em En
| MEDLINE
| ID: mdl-26452728
ABSTRACT
CONTEXT Citrus limon (L.) Burm.f. (Rutaceace) is a commonly available fruit variety with high medicinal and industrial values. OBJECTIVE:
Lemon peel (LP) extract was studied as a potent preventive and curative agent for experimentally induced hyperoxaluric rats. MATERIALS ANDMETHODS:
Gas chromatography-mass spectrometry (GC-MS) analyses and toxicity study were performed for aqueous methanol LP extract. Twenty-four Wistar rats were segregated into four groups. Group 1 Control; Group 2 Urolithic (ethylene glycol (EG) - 0.75%); Group 3 Preventive study (EG + LP extract administration from 0th to 7th week); Group 4 Curative study (EG + LP extract administration from 4th to 7th week). Animals received LP extract daily by oral administration (100 mg/kg body weight) for 7 weeks. RESULTS ANDDISCUSSION:
GC-MS analyses revealed that compound 6 was abundant in the LP extract (32%) followed by compound 1 (â¼21%). The LD50 value of LP extract was found to be >5000 mg/kg of body weight. Urolithic rats showed significantly higher urinary calcium and oxalate (4.47 ± 0.44 and 18.86 ± 0.55 mg/24 h, respectively) excretion compared with control and experimental rats. Renal function parameters like urea (84 ± 8.5 and 96.1 ± 3.6 mg/dL), creatinine (1.92 ± 0.27 and 1.52 ± 0.22 mg/dL), and urinary protein (2.03 ± 0.02 and 2.13 ± 0.16 mg/24 h) were also reduced by LP extract (p < 0.001) and corroborated with tissue analyses (SOD, catalase, and MDA levels) and histological studies in normal and experimental animals. Immunohistochemical staining of THP and NF-κB in urolithic animals showed elevated expression than the control, while LP extract suppressed the expression of these proteins.CONCLUSION:
In conclusion, lemon peel is effective in curing kidney stone disease and also can be used to prevent the disease and its recurrence.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Água
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Extratos Vegetais
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Citrus
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Metanol
/
Urolitíase
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Frutas
Limite:
Animals
Idioma:
En
Revista:
Pharm Biol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Índia