Myxococcus xanthus Growth, Development, and Isolation.
Curr Protoc Microbiol
; 39: 7A.1.1-7A.1.21, 2015 Nov 03.
Article
em En
| MEDLINE
| ID: mdl-26528785
ABSTRACT
Myxobacteria are a highly social group among the delta proteobacteria that display unique multicellular behaviors during their complex life cycle and provide a rare opportunity to study the boundary between single cells and multicellularity. These organisms are also unusual as their entire life cycle is surface associated and includes a number of social behaviors social gliding and rippling motility, 'wolf-pack'-like predation, and self-organizing complex biostructures, termed fruiting bodies, which are filled with differentiated environmentally resistant spores. Here we present methods for the growth, maintenance, and storage of Myxococcus xanthus, the most commonly studied of the myxobacteria. We also include methods to examine various developmental and social behaviors (fruiting body and spore formation, predation, and rippling motility). As the myxobacteria, similar to the streptomycetes, are excellent sources of many characterized and uncharacterized antibiotics and other natural products, we have provided a protocol for obtaining natural isolates from a variety of environmental sources.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação Biológica
/
Técnicas Microbiológicas
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Myxococcus xanthus
Idioma:
En
Revista:
Curr Protoc Microbiol
Ano de publicação:
2015
Tipo de documento:
Article