Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination.
J Clin Microbiol
; 54(1): 216-9, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26560537
ABSTRACT
During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Enzimas de Restrição do DNA
/
Descontaminação
/
Contaminação de Equipamentos
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Febre Aftosa
/
Reação em Cadeia da Polimerase em Tempo Real
/
Febre Aftosa
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2016
Tipo de documento:
Article