Probing the enzyme kinetics, allosteric modulation and activation of &#945;1- and &#945;2-subunit-containing AMP-activated protein kinase (AMPK) heterotrimeric complexes by pharmacological and physiological activators.

Rajamohan, Francis; Reyes, Allan R; Frisbie, Richard K; Hoth, Lise R; Sahasrabudhe, Parag; Magyar, Rachelle; Landro, James A; Withka, Jane M; Caspers, Nicole L; Calabrese, Matthew F; Ward, Jessica; Kurumbail, Ravi G

Probing the enzyme kinetics, allosteric modulation and activation of α1- and α2-subunit-containing AMP-activated protein kinase (AMPK) heterotrimeric complexes by pharmacological and physiological activators.

Rajamohan, Francis; Reyes, Allan R; Frisbie, Richard K; Hoth, Lise R; Sahasrabudhe, Parag; Magyar, Rachelle; Landro, James A; Withka, Jane M; Caspers, Nicole L; Calabrese, Matthew F; Ward, Jessica; Kurumbail, Ravi G.

Afiliação

Rajamohan F; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Reyes AR; CVMED Research unit, Pfizer Global Research and Development, 620 Memorial Drive, Cambridge, MA 02139, U.S.A.
Frisbie RK; The Department of Pharmacokinetics Dynamics and Metabolism, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Hoth LR; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Sahasrabudhe P; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Magyar R; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Landro JA; CVMED Research unit, Pfizer Global Research and Development, 620 Memorial Drive, Cambridge, MA 02139, U.S.A.
Withka JM; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Caspers NL; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Calabrese MF; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A.
Ward J; CVMED Research unit, Pfizer Global Research and Development, 620 Memorial Drive, Cambridge, MA 02139, U.S.A.
Kurumbail RG; Department of World Wide Medicinal Chemistry, Pfizer Global Research and Development, Eastern Point Road, Groton, CT 06340, U.S.A. ravi.g.kurumbail@pfizer.com.

Biochem J ; 473(5): 581-92, 2016 Mar 01.

Article em En | MEDLINE | ID: mdl-26635351

Assuntos

Proteínas Quinases Ativadas por AMP/química; Monofosfato de Adenosina/química; Regulação Alostérica; Compostos de Bifenilo; Ativação Enzimática; Ativadores de Enzimas/química; Ensaios Enzimáticos; Humanos; Isoenzimas/química; Cinética; Estrutura Terciária de Proteína; Subunidades Proteicas/química; Pironas/química; Proteínas Recombinantes/química; Tiofenos/química

Palavras-chave

AMP-activated protein kinase (AMPK); allosteric activation; heterotrimeric complex; kinase activity; metabolic sensor; recombinant protein

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Quinases Ativadas por AMP Limite: Humans Idioma: En Revista: Biochem J Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google