A transfected L-myc gene can substitute for c-myc in blocking murine erythroleukemia differentiation.
Mol Cell Biol
; 9(6): 2734-7, 1989 Jun.
Article
em En
| MEDLINE
| ID: mdl-2668739
ABSTRACT
We investigated the ability of the proto-oncogene L-myc to substitute for c-myc in blocking murine erythroleukemia differentiation. Murine erythroleukemia cells (line C19) were transfected with recombinant plasmids containing genomic and cDNA fragments of the L-myc gene driven by a Moloney murine leukemia virus long terminal repeat. Clones expressing constitutive high levels of L-myc failed to differentiate in response to the chemical inducer N,N'-hexamethylene bisacetamide (HMBA). The block to differentiation correlated with the level of L-myc expression. Furthermore, transfected clones grown in the presence of inducer for an extended period of time showed an increased level of L-myc expression. These results suggest that functional domains of the c-myc gene involved in differentiation are located in the discrete regions of homology between the c- and L-myc genes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proto-Oncogenes
/
Diferenciação Celular
/
Proteínas Proto-Oncogênicas
Limite:
Humans
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1989
Tipo de documento:
Article