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Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE(d)-T.
Taki, Masumi; Inoue, Hiroaki; Mochizuki, Kazuto; Yang, Jay; Ito, Yuji.
Afiliação
  • Taki M; Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC) , 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
  • Inoue H; Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC) , 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
  • Mochizuki K; Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC) , 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan.
  • Yang J; Department of Anesthesiology, University of Wisconsin, School of Medicine and Public Health , Madison, Wisconsin 53706 United States.
  • Ito Y; Department of Chemistry and Bioscience, Graduate School of Science and Engineering, Kagoshima University , 1-21-35 Korimoto, Kagoshima, Kagoshima 890-0065, Japan.
Anal Chem ; 88(2): 1096-9, 2016 Jan 19.
Article em En | MEDLINE | ID: mdl-26727351
ABSTRACT
To obtain a molecular probe for specific protein detection, we have synthesized fluorogenic probe library of vast diversity on bacteriophage T7 via the gp10 based-thioetherificaion (10BASE(d)-T). A remarkable color-changing and turning-on probe was selected from the library, and its physicochemical properties upon target-specific binding were obtained. Combination analyses of fluorescence emission titration, isothermal titration calorimetry (ITC), and quantitative saturation-transfer difference (STD) NMR measurements, followed by in silico docking simulation, rationalized the most plausible geometry of the ligand-protein interaction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos de Sulfidrila / Proteínas Virais / Sondas Moleculares / Bacteriófago T7 / Cor / Éteres / Corantes Fluorescentes Idioma: En Revista: Anal Chem Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Japão
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos de Sulfidrila / Proteínas Virais / Sondas Moleculares / Bacteriófago T7 / Cor / Éteres / Corantes Fluorescentes Idioma: En Revista: Anal Chem Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Japão