Tissue Dissociation for Metastasis Studies.
Cold Spring Harb Protoc
; 2016(2): pdb.prot078329, 2016 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-26832679
ABSTRACT
The main requirement for most metastasis-related applications is the conversion of solid tissue into a single-cell suspension. In theory, this suspension represents the diversity of cells present in the tissue, whether malignant or benign. We have found that cell viability, as measured by trypan blue staining or fluorescence-activated cell sorting (FACS), is critical for evaluating the success of the tissue-dissociation procedure. The recommended goal is at least 70% cell viability.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Metástase Neoplásica
Limite:
Animals
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Canadá