Detection of Autophagy in Caenorhabditis elegans.
Cold Spring Harb Protoc
; 2016(2): pdb.top070466, 2016 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-26832690
ABSTRACT
Autophagy is a dynamic and catabolic process that results in the breakdown and recycling of cellular components through the autophagosomal-lysosomal pathway. Many autophagy genes identified in yeasts and mammals have orthologs in the nematode Caenorhabditis elegans. In recent years, gene inactivation by RNA interference (RNAi) and chromosomal mutations has been useful to probe the functions of autophagy in C. elegans, and a role for autophagy has been shown to contribute to multiple processes, such as the adaptation to stress, longevity, cell death, cell growth control, clearance of aggregation-prone proteins, degradation of P granules during embryogenesis, and apoptotic cell clearance. Here, we discuss some of these roles and describe methods that can be used to study autophagy in C. elegans. Specifically, we summarize how to visualize autophagy in embryos, larva, or adults, how to detect the lipidation of the ubiquitin-like modifier LGG-1 by western blot, and how to inactivate autophagy genes by RNAi.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Parasitologia
/
Autofagia
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Caenorhabditis elegans
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Imagem Óptica
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Biologia Molecular
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2016
Tipo de documento:
Article