Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain.
Nat Methods
; 13(4): 325-8, 2016 Apr.
Article
em En
| MEDLINE
| ID: mdl-26878381
ABSTRACT
Real-time activity measurements from multiple specific cell populations and projections are likely to be important for understanding the brain as a dynamical system. Here we developed frame-projected independent-fiber photometry (FIP), which we used to record fluorescence activity signals from many brain regions simultaneously in freely behaving mice. We explored the versatility of the FIP microscope by quantifying real-time activity relationships among many brain regions during social behavior, simultaneously recording activity along multiple axonal pathways during sensory experience, performing simultaneous two-color activity recording, and applying optical perturbation tuned to elicit dynamics that match naturally occurring patterns observed during behavior.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fotometria
/
Comportamento Social
/
Encéfalo
/
Mapeamento Encefálico
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Sinalização do Cálcio
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Vias Neurais
Limite:
Animals
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos