Targeted Ultrasound-Assisted Cancer-Selective Chemical Labeling and Subsequent Cancer Imaging using Click Chemistry.
Angew Chem Int Ed Engl
; 55(18): 5452-6, 2016 04 25.
Article
em En
| MEDLINE
| ID: mdl-27010510
ABSTRACT
Metabolic sugar labeling followed by the use of reagent-free click chemistry is an established technique for inâ
vitro cell targeting. However, selective metabolic labeling of the target tissues inâ
vivo remains a challenge to overcome, which has prohibited the use of this technique for targeted inâ
vivo applications. Herein, we report the use of targeted ultrasound pulses to induce the release of tetraacetyl N-azidoacetylmannosamine (Ac4 ManAz) from microbubbles (MBs) and its metabolic expression in the cancer area. Ac4 ManAz-loaded MBs showed great stability under physiological conditions, but rapidly collapsed in the presence of tumor-localized ultrasound pulses. The released Ac4 ManAz from MBs was able to label 4T1 tumor cells with azido groups and significantly improved the tumor accumulation of dibenzocyclooctyne (DBCO)-Cy5 by subsequent click chemistry. We demonstrated for the first time that Ac4 ManAz-loaded MBs coupled with the use of targeted ultrasound could be a simple but powerful tool for inâ
vivo cancer-selective labeling and targeted cancer therapies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Azidas
/
Neoplasias da Mama
/
Sistemas de Liberação de Medicamentos
/
Microbolhas
/
Química Click
/
Hexosaminas
Limite:
Animals
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Estados Unidos