Red fluorescent biofilm: the thick, the old, and the cariogenic.
J Oral Microbiol
; 8: 30346, 2016.
Article
em En
| MEDLINE
| ID: mdl-27060056
ABSTRACT
BACKGROUND:
Some dental plaque fluoresces red. The factors involved in this fluorescence are yet unknown.OBJECTIVE:
The aim of this study was to assess systematically the effect of age, thickness, and cariogenicity on the extent of red fluorescence produced by in vitro microcosm biofilms.DESIGN:
The effects of biofilm age and thickness on red fluorescence were tested in a constant depth film fermentor (CDFF) by growing biofilms of variable thicknesses that received a constant supply of defined mucin medium (DMM) and eight pulses of sucrose/day. The influence of cariogenicity on red fluorescence was tested by growing biofilm on dentin disks receiving DMM, supplemented with three or eight pulses of sucrose/day. The biofilms were analyzed at different time points after inoculation, up to 24 days. Emission spectra were measured using a fluorescence spectrophotometer (λexc405 nm) and the biofilms were photographed with a fluorescence camera. The composition of the biofilms was assessed using 454-pyrosequecing of the 16S rDNA gene.RESULTS:
From day 7 onward, the biofilms emitted increasing intensities of red fluorescence as evidenced by the combined red fluorescence peaks. The red fluorescence intensity correlated with biofilm thickness but not in a linear way. Biofilm fluorescence also correlated with the imposed cariogenicity, evidenced by the induced dentin mineral loss. Increasing the biofilm age or increasing the sucrose pulsing frequency led to a shift in the microbial composition. These shifts in composition were accompanied by an increase in red fluorescence.CONCLUSIONS:
The current study shows that a thicker, older, or more cariogenic biofilm results in a higher intensity of red fluorescence.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Oral Microbiol
Ano de publicação:
2016
Tipo de documento:
Article