Enzyme immobilization on a nanoadsorbent for improved stability against heavy metal poisoning.
Colloids Surf B Biointerfaces
; 144: 135-142, 2016 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-27085045
ABSTRACT
Magnetic nanoparticles modified with siloxane layers bearing amino and thiol functions have been used for immobilization of urease either by adsorption or via surface grafting. The activity of the immobilized enzyme in the hydrolysis of urea extended to the levels typical of the native enzyme, while its long-term stability in combination with magnetic retraction opened for its repeated use in both analysis and detoxification of bio-fluids. The immobilized urease revealed strongly enhanced stability and 65% activity in the presence of 0.1mmol/l of Hg(2+) or 0.3mmol/l of Cu(2+) while the native urease did not retain any activity at all. The enzyme grafting was shown to be a potentially perspective tool in alleviation of heavy metal poisoning and to be providing an opportunity for use of the developed adsorbents as both biosensors and bio-reactants for removal of urea from biofluids.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Intoxicação
/
Urease
/
Enzimas Imobilizadas
/
Nanopartículas de Magnetita
/
Intoxicação por Metais Pesados
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Assunto da revista:
QUIMICA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Ucrânia