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Relative Rates of Surface and Volume Synthesis Set Bacterial Cell Size.
Harris, Leigh K; Theriot, Julie A.
Afiliação
  • Harris LK; Biophysics Program, Department of Biochemistry and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Theriot JA; Biophysics Program, Department of Biochemistry and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: theriot@stanford.edu.
Cell ; 165(6): 1479-1492, 2016 Jun 02.
Article em En | MEDLINE | ID: mdl-27259152
Many studies have focused on the mechanisms underlying length and width determination in rod-shaped bacteria. Here, we focus instead on cell surface area to volume ratio (SA/V) and demonstrate that SA/V homeostasis underlies size determination. We propose a model whereby the instantaneous rates of surface and volume synthesis both scale with volume. This model predicts that these relative rates dictate SA/V and that cells approach a new steady-state SA/V exponentially, with a decay constant equal to the volume growth rate. To test this, we exposed diverse bacterial species to sublethal concentrations of a cell wall biosynthesis inhibitor and observed dose-dependent decreases in SA/V. Furthermore, this decrease was exponential and had the expected decay constant. The model also quantitatively describes SA/V alterations induced by other chemical, nutritional, and genetic perturbations. We additionally present evidence for a surface material accumulation threshold underlying division, sensitizing cell length to changes in SA/V requirements.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias Tipo de estudo: Prognostic_studies Idioma: En Revista: Cell Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias Tipo de estudo: Prognostic_studies Idioma: En Revista: Cell Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos