New Genetic Constructs for Generation of Stable Therapeutic Antibodies to Organophosphorus Toxins in Methylotrophic Yeasts Pichia Pastoris.
Bull Exp Biol Med
; 161(1): 83-7, 2016 May.
Article
em En
| MEDLINE
| ID: mdl-27270933
ABSTRACT
We propose a new method of obtaining of stable Fab-fragments of antibodies in Pichia pastoris expression system. Recently, we obtained Fab-fragments of antibodies neutralizing organophosphorus toxins. However, high yield of the target products was not attained because of high level of proteolytic degradation. In the present study, we identified sites of proteolytic degradation in Fab-fragments and endogenous proteases performing degradation, which allowed obtaining optimized genetic constructs for expression of antibody heavy chains (IgGγ1) and kappa and lambda isotypes of light chains. Co-transformation of these vectors allowed obtaining Fab-fragments of antibodies to organophosphorus toxins without proteolytic degradation of the product.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Organofosforados
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Pichia
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Fragmentos Fab das Imunoglobulinas
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Anticorpos Catalíticos
Idioma:
En
Revista:
Bull Exp Biol Med
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Federação Russa