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SUMOylation of DNA topoisomerase IIα regulates histone H3 kinase Haspin and H3 phosphorylation in mitosis.
Yoshida, Makoto M; Ting, Lily; Gygi, Steven P; Azuma, Yoshiaki.
Afiliação
  • Yoshida MM; Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045.
  • Ting L; Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
  • Gygi SP; Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
  • Azuma Y; Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045 azumay@ku.edu.
J Cell Biol ; 213(6): 665-78, 2016 06 20.
Article em En | MEDLINE | ID: mdl-27325792
ABSTRACT
DNA topoisomerase II (TOP2) plays a pivotal role in faithful chromosome separation through its strand-passaging activity that resolves tangled genomic DNA during mitosis. Additionally, TOP2 controls progression of mitosis by activating cell cycle checkpoints. Recent work showed that the enzymatically inert C-terminal domain (CTD) of TOP2 and its posttranslational modification are critical to this checkpoint regulation. However, the molecular mechanism has not yet been determined. By using Xenopus laevis egg extract, we found that SUMOylation of DNA topoisomerase IIα (TOP2A) CTD regulates the localization of the histone H3 kinase Haspin and phosphorylation of histone H3 at threonine 3 at the centromere, two steps known to be involved in the recruitment of the chromosomal passenger complex (CPC) to kinetochores in mitosis. Robust centromeric Haspin localization requires SUMOylated TOP2A CTD binding activity through SUMO-interaction motifs and the phosphorylation of Haspin. We propose a novel mechanism through which the TOP2 CTD regulates the CPC via direct interaction with Haspin at mitotic centromeres.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosforilação / Histonas / DNA Topoisomerases Tipo II / Proteínas de Ligação a DNA / Sumoilação / Mitose / Antígenos de Neoplasias Limite: Animals Idioma: En Revista: J Cell Biol Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosforilação / Histonas / DNA Topoisomerases Tipo II / Proteínas de Ligação a DNA / Sumoilação / Mitose / Antígenos de Neoplasias Limite: Animals Idioma: En Revista: J Cell Biol Ano de publicação: 2016 Tipo de documento: Article