Derivation of Huntington Disease affected Genea018 human embryonic stem cell line.
Stem Cell Res
; 16(2): 423-6, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-27346005
The Genea018 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 46 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 75% of cells expressed Nanog, 91% Oct4, 73% Tra1-60 and 96% SSEA4, gave a Pluritest pluripotency score of 31.12, Novelty of 1.45, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Doença de Huntington
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Células-Tronco Embrionárias Humanas
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Austrália