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Real-time monitoring of amyloid growth in a rigid gel matrix.
Dalpadado, Roshan C; Maat, Hendrik; Carver, John A; Hall, Damien.
Afiliação
  • Dalpadado RC; Research School of Chemistry, Australian National University, Acton ACT 2601, Australia.
  • Maat H; Research School of Chemistry, Australian National University, Acton ACT 2601, Australia.
  • Carver JA; Research School of Chemistry, Australian National University, Acton ACT 2601, Australia.
  • Hall D; Research School of Chemistry, Australian National University, Acton ACT 2601, Australia; Institute for Protein Research Osaka University, 3-1- Yamada-oka, Suita, Osaka 565-0871, Japan. Electronic address: damien.hall@anu.edu.au.
Anal Biochem ; 511: 13-6, 2016 10 15.
Article em En | MEDLINE | ID: mdl-27477869
We demonstrate the real-time monitoring of the growth of amyloid-protein aggregates in a semi-rigid gel environment constructed from a 5% w/v gelatin solution. The kinetics of amyloid fibril growth from reduced and carboxy-methylated κ-casein occurring in the gel medium was contrasted against that obtained in a regular solution assay. Aggregation kinetics were recorded using Thioflavin T fluorescence. Transmission electron microscopy was used to confirm the aggregates' existence and morphology. The current demonstration of controlled amyloid growth in a gel environment represents the first step towards development of an experimental model for investigating the role of spatial and medium factors in the kinetics of aggregation-based proteopathies.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tiazóis / Caseínas / Agregação Patológica de Proteínas / Gelatina / Amiloide Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Anal Biochem Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tiazóis / Caseínas / Agregação Patológica de Proteínas / Gelatina / Amiloide Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Anal Biochem Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Austrália