Evaluation of the Impact of Methicillin-Resistant Staphylococcus pseudintermedius Biofilm Formation on Antimicrobial Susceptibility.
Vet Surg
; 45(7): 968-971, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-27500604
ABSTRACT
OBJECTIVE:
To compare the minimum inhibitory concentration (MIC) of four antimicrobials in planktonic vs. biofilm-associated Staphylococcus pseudintermedius. STUDYDESIGN:
In vitro study. SAMPLE POPULATION 78 isolates from dogs colonized or infected with methicillin-resistant S. pseudintermedius (MRSP, n=39) or methicillin-susceptible S. pseudintermedius (MSSP, n=39).METHODS:
Agar dilution was used to determine the MIC of amikacin, cefazolin, enrofloxacin, and gentamicin for planktonic bacteria. A modified broth microdilution assay was used to assess the MIC of biofilm-associated bacteria.RESULTS:
MIC were significantly higher in biofilm-associated vs. planktonic bacteria for all antimicrobials; amikacin (median MIC biofilm >2,000 µg/mL vs. planktonic 3 µg/mL, P<.0001), cefazolin (>1,000 vs. 0.5 µg/mL, P<.0001), enrofloxacin (>1,000 vs. 0.25 µg/mL, P<.0001), and gentamicin (>1,000 vs. 0.3 µg/mL, P<.001). For all antimicrobials, there were significant differences in planktonic MIC for MRSP and MSSP (all P<.0001) but no differences between biofilm MIC for MRSP and MSSP (P=.08-1.0).CONCLUSION:
The MIC for biofilm-associated S. pseudintermedius are significantly higher than for planktonic bacteria. Standard methods for determining MIC are not appropriate for biofilm-associated infections. This must be considered when determining treatment regimens for infections that potentially involve biofilms, and further study of methods to control biofilm-associated infections is needed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plâncton
/
Infecções Estafilocócicas
/
Staphylococcus
/
Biofilmes
/
Doenças do Cão
/
Antibacterianos
Limite:
Animals
País/Região como assunto:
America do norte
Idioma:
En
Revista:
Vet Surg
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Canadá