Optimized expression of prolyl aminopeptidase in Pichia pastoris and its characteristics after glycosylation.

Yang, Hongyu; Zhu, Qiang; Zhou, Nandi; Tian, Yaping

Yang, Hongyu; Zhu, Qiang; Zhou, Nandi; Tian, Yaping.

Afiliação

Yang H; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Zhu Q; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Zhou N; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Tian Y; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. biochem@jiangnan.edu.cn.

World J Microbiol Biotechnol ; 32(11): 176, 2016 Nov.

Article em En | MEDLINE | ID: mdl-27628336

Assuntos

Aminopeptidases/genética; Aminopeptidases/metabolismo; Aspergillus oryzae/enzimologia; Pichia/genética; Aspergillus oryzae/genética; Clonagem Molecular; Proteínas Fúngicas/genética; Proteínas Fúngicas/metabolismo; Glicosilação; Concentração de Íons de Hidrogênio; Peso Molecular; Pichia/crescimento & desenvolvimento; Proteínas Recombinantes; Especificidade por Substrato; Temperatura

Palavras-chave

N-glycosylation; Pichia pastoris; Prolyl aminopeptidase; Thermostability

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pichia / Aspergillus oryzae / Aminopeptidases Idioma: En Revista: World J Microbiol Biotechnol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China

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