Estimation of Intra-vitreal Half-Lifes in the Rabbit Eye with Semi-mechanistic Equations.
Pharm Res
; 34(1): 49-57, 2017 01.
Article
em En
| MEDLINE
| ID: mdl-27628626
ABSTRACT
PURPOSE:
To develop an alternative method for estimating vitreal half-lifes in the rabbit eye based on simple equations for the physical processes of dissipation and the physiochemical properties of therapeutic substances applied by intravitreal drug administration.METHODS:
Equations were derived to describe diffusion in the vitreous humor and permeation through the back-of-the-eye tissue, and the volume of distribution. The model was validated using reported half-life values from 83 compounds collected from literature.RESULTS:
The rate limiting step for dissipation from the vitreous depends mainly on the molecular weight. Dissipation of very low molecular weight (MW) substances (<350 Da) is limited by diffusional transport to the back of the eye, for substances with a MW >350 Da uptake into the back of the eye tissue becomes limiting, and large molecules >500 Da predominantly take an alternative path being cleared through the front of the eye for which diffusion towards the posterior chamber turns out to be limiting. Taking the three rate determining processes into account, the derived model can estimate dissipation rates and respectively vitreal half-life values of small compounds and macromolecules from their molecular weight with very few exceptions.CONCLUSIONS:
The equations derived in this analysis provide a simple method to predict vitreal half-lifes for a diverse group of molecules and can be easily implemented in early drug development.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Corpo Vítreo
/
Preparações Farmacêuticas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Pharm Res
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha