Proteomics analysis of the endogenous, constitutive, leaf SUMOylome.
J Proteomics
; 150: 268-280, 2017 01 06.
Article
em En
| MEDLINE
| ID: mdl-27671789
ABSTRACT
SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. SIGNIFICANCE:
The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Solanum tuberosum
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Processamento de Proteína Pós-Traducional
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Folhas de Planta
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Proteômica
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Sumoilação
Idioma:
En
Revista:
J Proteomics
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Bélgica