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The Salivary Protein Repertoire of the Polyphagous Spider Mite Tetranychus urticae: A Quest for Effectors.
Jonckheere, Wim; Dermauw, Wannes; Zhurov, Vladimir; Wybouw, Nicky; Van den Bulcke, Jan; Villarroel, Carlos A; Greenhalgh, Robert; Grbic, Mike; Schuurink, Rob C; Tirry, Luc; Baggerman, Geert; Clark, Richard M; Kant, Merijn R; Vanholme, Bartel; Menschaert, Gerben; Van Leeuwen, Thomas.
Afiliação
  • Jonckheere W; From the ‡Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Gent, Belgium.
  • Dermauw W; §Department of Evolutionary Biology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.
  • Zhurov V; From the ‡Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Gent, Belgium; wannes.dermauw@ugent.be gerben.menschaert@UGent.be thomas.vanleeuwen@ugent.be.
  • Wybouw N; ¶Department of Biology, The University of Western Ontario, London, ON, Canada N6A5B7.
  • Van den Bulcke J; §Department of Evolutionary Biology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.
  • Villarroel CA; ‖UGCT - Woodlab-UGent, Department of Forest and Water Management, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Gent, Belgium.
  • Greenhalgh R; **Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.
  • Grbic M; ‡‡Department of Population Biology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.
  • Schuurink RC; §§Department of Biology, University of Utah, Salt Lake City 257 South 1400 East Utah 84112.
  • Tirry L; ¶Department of Biology, The University of Western Ontario, London, ON, Canada N6A5B7.
  • Baggerman G; ¶¶Instituto de Ciencias de la Vid y el Vino, 26006 Logrono, Spain.
  • Clark RM; **Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands.
  • Kant MR; From the ‡Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Gent, Belgium.
  • Vanholme B; ‖‖Center for Proteomics (CFP), University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.
  • Menschaert G; Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium.
  • Van Leeuwen T; §§Department of Biology, University of Utah, Salt Lake City 257 South 1400 East Utah 84112.
Mol Cell Proteomics ; 15(12): 3594-3613, 2016 Dec.
Article em En | MEDLINE | ID: mdl-27703040
The two-spotted spider mite Tetranychus urticae is an extremely polyphagous crop pest. Alongside an unparalleled detoxification potential for plant secondary metabolites, it has recently been shown that spider mites can attenuate or even suppress plant defenses. Salivary constituents, notably effectors, have been proposed to play an important role in manipulating plant defenses and might determine the outcome of plant-mite interactions. Here, the proteomic composition of saliva from T. urticae lines adapted to various host plants-bean, maize, soy, and tomato-was analyzed using a custom-developed feeding assay coupled with nano-LC tandem mass spectrometry. About 90 putative T. urticae salivary proteins were identified. Many are of unknown function, and in numerous cases belonging to multimembered gene families. RNAseq expression analysis revealed that many genes coding for these salivary proteins were highly expressed in the proterosoma, the mite body region that includes the salivary glands. A subset of genes encoding putative salivary proteins was selected for whole-mount in situ hybridization, and were found to be expressed in the anterior and dorsal podocephalic glands. Strikingly, host plant dependent expression was evident for putative salivary proteins, and was further studied in detail by micro-array based genome-wide expression profiling. This meta-analysis revealed for the first time the salivary protein repertoire of a phytophagous chelicerate. The availability of this salivary proteome will assist in unraveling the molecular interface between phytophagous mites and their host plants, and may ultimately facilitate the development of mite-resistant crops. Furthermore, the technique used in this study is a time- and resource-efficient method to examine the salivary protein composition of other small arthropods for which saliva or salivary glands cannot be isolated easily.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas e Peptídeos Salivares / Produtos Agrícolas / Tetranychidae / Proteômica Tipo de estudo: Systematic_reviews Limite: Animals Idioma: En Revista: Mol Cell Proteomics Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Bélgica

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas e Peptídeos Salivares / Produtos Agrícolas / Tetranychidae / Proteômica Tipo de estudo: Systematic_reviews Limite: Animals Idioma: En Revista: Mol Cell Proteomics Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Bélgica