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Evaluation of Long-Term Cryostorage of Brain Tissue Sections for Quantitative Histochemistry.
Estrada, Larissa I; Robinson, Amy A; Amaral, Ana C; Giannaris, Eustathia L; Heyworth, Nadine C; Mortazavi, Farzad; Ngwenya, Laura B; Roberts, Debra E; Cabral, Howard J; Killiany, Ronald J; Rosene, Douglas L.
Afiliação
  • Estrada LI; Department of Pharmacology and Experimental Therapeutics (LIE, AAR).
  • Robinson AA; Department of Pharmacology and Experimental Therapeutics (LIE, AAR).
  • Amaral AC; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
  • Giannaris EL; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
  • Heyworth NC; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
  • Mortazavi F; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
  • Ngwenya LB; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
  • Roberts DE; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
  • Cabral HJ; Boston University School of Medicine, Boston, Massachusetts, and Department of Biostatistics, Boston University School of Public Health, Boston, Massachusetts (HJC).
  • Killiany RJ; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
  • Rosene DL; Department of Anatomy and Neurobiology (ACA, ELG, NCH, FM, LBN, DER, RJK, DLR).
J Histochem Cytochem ; 65(3): 153-171, 2017 03.
Article em En | MEDLINE | ID: mdl-28080173
Storage of tissue sections for long periods allows multiple samples, acquired over months or years, to be processed together, in the same reagents, for quantitative histochemical studies. Protocols for freezer storage of free-floating frozen sections using sucrose with different additives have been reported and assert that storage has no effect on histochemistry, but no quantitative support has been provided. The present study analyzed the efficacy of long-term storage of brain tissue sections at -80C in buffered 15% glycerol. To determine whether histochemical reactivity is affected, we analyzed 11 datasets from 80 monkey brains that had sections stored for up to 10 years. For processing, sections from multiple cases were removed from storage, thawed, and batch-processed at the same time for different histochemical measures, including IHC for neuronal nuclear antigen, parvalbumin, orexin-A, doublecortin, bromodeoxyuridine, the pro-form of brain-derived neurotrophic factor, and damaged myelin basic protein as well as a histochemical assay for hyaluronic acid. Results were quantified using stereology, optical densitometry, fluorescence intensity, or percent area stained. Multiple regression analyses controlling for age and sex demonstrated the general stability of these antigens for up to a decade when stored in 15% glycerol at -80C.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Química Encefálica / Secções Congeladas Limite: Animals Idioma: En Revista: J Histochem Cytochem Assunto da revista: HISTOCITOQUIMICA Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Química Encefálica / Secções Congeladas Limite: Animals Idioma: En Revista: J Histochem Cytochem Assunto da revista: HISTOCITOQUIMICA Ano de publicação: 2017 Tipo de documento: Article