Phosphatidylserine synthesis at membrane contact sites promotes its transport out of the ER.
J Lipid Res
; 58(3): 553-562, 2017 03.
Article
em En
| MEDLINE
| ID: mdl-28119445
ABSTRACT
Close contacts between organelles, often called membrane contact sites (MCSs), are regions where lipids are exchanged between organelles. Here, we identify a novel mechanism by which cells promote phospholipid exchange at MCSs. Previous studies have shown that phosphatidylserine (PS) synthase activity is highly enriched in portions of the endoplasmic reticulum (ER) in contact with mitochondria. The objective of this study was to determine whether this enrichment promotes PS transport out of the ER. We found that PS transport to mitochondria was more efficient when PS synthase was fused to a protein in the ER at ER-mitochondria contacts than when it was fused to a protein in all portions of the ER. Inefficient PS transport to mitochondria was corrected by increasing tethering between these organelles. PS transport to endosomes was similarly enhanced by PS production in regions of the ER in contact with endosomes. Together, these findings indicate that PS production at MCSs promotes PS transport out of the ER and suggest that phospholipid production at MCSs may be a general mechanism of channeling lipids to specific cellular compartments.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilserinas
/
CDPdiacilglicerol-Serina O-Fosfatidiltransferase
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Proteínas de Saccharomyces cerevisiae
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Retículo Endoplasmático
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Metabolismo dos Lipídeos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Lipid Res
Ano de publicação:
2017
Tipo de documento:
Article