The effect of additive compounds on glycerol-induced damage to human chondrocytes.
Cryobiology
; 75: 68-74, 2017 04.
Article
em En
| MEDLINE
| ID: mdl-28192075
ABSTRACT
High concentrations of cryoprotective agents are required for cryopreservation techniques such as vitrification. Glycerol is a common cryoprotective agent used in cryopreservation protocols but this agent is toxic at high concentrations. This work is an attempt to mitigate the toxic effects of high concentrations of glycerol on intact chondrocytes in human knee articular cartilage from total knee arthroplasty patients by simultaneous exposure to glycerol and a variety of additive compounds. The resulting cell viability in the cartilage samples as measured by membrane integrity staining showed that, in at least one concentration or in combination, all of the tested additive compounds (tetramethylpyrazine, ascorbic acid, chondroitin sulphate, glucosamine sulphate) were able to reduce the deleterious effects of glycerol exposure when examination of membrane integrity took place on a delayed time frame. The use of additive compounds to reduce cryoprotectant toxicity in articular cartilage may help improve cell recovery after cryopreservation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Condrócitos
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Crioprotetores
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Glicerol
Limite:
Aged
/
Aged80
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Humans
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Middle aged
Idioma:
En
Revista:
Cryobiology
Ano de publicação:
2017
Tipo de documento:
Article