High throughput selection of antibiotic-resistant transgenic Arabidopsis plants.
Anal Biochem
; 525: 44-45, 2017 05 15.
Article
em En
| MEDLINE
| ID: mdl-28249723
ABSTRACT
Kanamycin resistance is the most frequently used antibiotic-resistance marker for Arabidopsis transformations, however, this method frequently causes escape of untransformed plants, particularly at the high seedling density during the selection. Here we developed a robust high-density selection method using top agar for Arabidopsis thaliana. Top agar effectively suppressed growth of untransformed wild-type plants on selection media at high density. Survival of the transformed plants during the selection were confirmed by production of green true leaves and expression of a firefly luciferase reporter gene. Top agar method allowed selection using a large amount of seeds in Arabidopsis transformation.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência a Canamicina
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Engenharia Genética
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Plantas Geneticamente Modificadas
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Arabidopsis
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Folhas de Planta
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Ágar
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Antibacterianos
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2017
Tipo de documento:
Article