RNA interference knockdown of aminopeptidase N genes decrease the susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca-expressing transgenic rice.

Qiu, Lin; Fan, Jinxing; Zhang, Boyao; Liu, Lang; Wang, Xiaoping; Lei, Chaoliang; Lin, Yongjun; Ma, Weihua

Qiu, Lin; Fan, Jinxing; Zhang, Boyao; Liu, Lang; Wang, Xiaoping; Lei, Chaoliang; Lin, Yongjun; Ma, Weihua.

Afiliação

Qiu L; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Fan J; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Zhang B; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Liu L; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Wang X; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Lei C; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Lin Y; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China.
Ma W; National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address

J Invertebr Pathol ; 145: 9-12, 2017 05.

Article em En | MEDLINE | ID: mdl-28274717

RESUMO

Transgenic rice expressing Bacillus thuringiensis (Bt) Cry toxins are resistant to lepidopteran pests, such as Chilo suppressalis, a major insect pest of rice in Asia. Understanding how these toxins interact with their hosts is crucial to understanding their insecticidal action. In this study, knockdown of two aminopeptidase N genes (APN1 and APN2) by RNA interference resulted in decreased susceptibility of C. suppressalis larvae to the Bt rice varieties TT51 (Cry1Ab and Cry1Ac fusion genes) and T1C-19 (Cry1Ca), but not T2A-1 (Cry2Aa). This suggests that APN1 and APN2 are receptors for Cry1A and Cry1C toxins in C. suppressalis.

Assuntos

Lepidópteros/genética; Oryza/microbiologia; Controle Biológico de Vetores/métodos; Plantas Geneticamente Modificadas/genética; Animais; Toxinas de Bacillus thuringiensis; Proteínas de Bactérias/genética; Endotoxinas/genética; Técnicas de Silenciamento de Genes; Genes de Insetos/genética; Proteínas Hemolisinas/genética; Larva; Oryza/genética

Palavras-chave

Aminopeptidase N; Bacillus thuringiensis; Bt receptor; Chilo suppressalis; Cry toxin; RNA interference

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Controle Biológico de Vetores / Plantas Geneticamente Modificadas / Lepidópteros Limite: Animals Idioma: En Revista: J Invertebr Pathol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

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