Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis.
Biochem Biophys Res Commun
; 486(3): 804-810, 2017 05 06.
Article
em En
| MEDLINE
| ID: mdl-28351618
ABSTRACT
Tuberculosis (TB) is a severe disease caused by Mycobacterium tuberculosis (M. tb) and the well-characterized M. tb MazE/F proteins play important roles in stress adaptation. Recently, the MazF-mt9 toxin has been found to display endonuclease activities towards tRNAs but the mechanism is unknown. We hereby present the crystal structure of apo-MazF-mt9. The enzyme recognizes tRNALys with a central UUU motif within the anticodon loop, but is insensitive to the sequence context outside of the loop. Based on our crystallographic and biochemical studies, we identified key residues for catalysis and proposed the potential tRNA-binding site.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoproteínas
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Proteínas de Bactérias
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Toxinas Bacterianas
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Anticódon
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RNA de Transferência de Lisina
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Endorribonucleases
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Mycobacterium tuberculosis
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2017
Tipo de documento:
Article