Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum.

Blondal, Thorarinn; Brunetto, Maurizia Rossana; Cavallone, Daniela; Mikkelsen, Martin; Thorsen, Michael; Mang, Yuan; Pinheiro, Hazel; Bonino, Ferruccio; Mouritzen, Peter

Blondal, Thorarinn; Brunetto, Maurizia Rossana; Cavallone, Daniela; Mikkelsen, Martin; Thorsen, Michael; Mang, Yuan; Pinheiro, Hazel; Bonino, Ferruccio; Mouritzen, Peter.

Afiliação

Blondal T; Exiqon A/S, Vedbaek, Denmark.
Brunetto MR; Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Pisa, Italy.
Cavallone D; Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Pisa, Italy.
Mikkelsen M; Exiqon A/S, Vedbaek, Denmark.
Thorsen M; Exiqon A/S, Vedbaek, Denmark.
Mang Y; Exiqon A/S, Vedbaek, Denmark.
Pinheiro H; Exiqon A/S, Vedbaek, Denmark.
Bonino F; Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Pisa, Italy.
Mouritzen P; Exiqon A/S, Vedbaek, Denmark. pm@exiqon.com.

Methods Mol Biol ; 1580: 21-44, 2017.

Article em En | MEDLINE | ID: mdl-28439824

ABSTRACT

ABSTRACT

This study compares next-generation sequencing (NGS) technologies that have been optimized specifically for biofluid samples, with more established qPCR-based methods for profiling microRNAs in biofluids. The same patient serum samples were analyzed by NGS and qPCR, and differences in the serum microRNA profile between HBV and HCV infected patients were investigated. While there was overall good agreement between NGS and qPCR, there were some differences between the platforms, highlighting the importance of validation.

Assuntos

Perfilação da Expressão Gênica/métodos; Sequenciamento de Nucleotídeos em Larga Escala/métodos; MicroRNAs/sangue; MicroRNAs/genética; Hepacivirus/isolamento & purificação; Hepatite B/sangue; Hepatite B/genética; Vírus da Hepatite B/isolamento & purificação; Hepatite C/sangue; Hepatite C/genética; Humanos; Reação em Cadeia da Polimerase em Tempo Real/métodos; Reprodutibilidade dos Testes

Palavras-chave

Biofluids; HBV; HCV; Liquid biopsy; NGS; Next-generation sequencing; Plasma; Platform comparison; Profiling; Real-time PCR; Serum; Validation; miRNA; microRNA; qPCR

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Perfilação da Expressão Gênica / MicroRNAs / Sequenciamento de Nucleotídeos em Larga Escala Limite: Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Dinamarca

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