Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary.
Balkan Med J
; 34(5): 444-449, 2017 Sep 29.
Article
em En
| MEDLINE
| ID: mdl-28443590
ABSTRACT
BACKGROUND:
Apigenin is a plant-derived compound belonging to the flavone class, which possess antioxidant, free-radical-scavenging and anti-inflammatory properties.AIMS:
To address the effects of apigenin on serum anti-mullerian hormone levels, tissue oxidative stress parameters and histopathological changes in ovarian ischemia/reperfusion injury. STUDYDESIGN:
Animal experiment.METHODS:
Twenty-eight female Wistar albino rats were randomly separated into four sections Sham operation (group 1), ischemia/reperfusion plus saline (group 2), ischemia/reperfusion plus dimethyl sulfoxide (group 3) and ischemia/reperfusion plus apigenin (group 4). In all ischemia/reperfusion groups, a bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion. A single dose of 15 mg/kg apigenin was given intraperitoneally 60 min before reperfusion in group 4. After 3-h of reperfusion, both ovaries were removed, and blood samples were collected. The main outcome measures were serum anti-mullerian hormone levels, ovarian tissue malondialdehyde, total nitric oxide, Cu/Zn superoxide dismutase, catalase and glutathione levels and histopathological damage scores.RESULTS:
The ovarian tissue nitric oxide level was significantly lower, and the glutathione level was significantly higher in group 4 compared with groups 2 and 3. There was no significant difference in anti-mullerian hormone levels among the three ischemia/reperfusion groups. The histopathological damage score was lower in group 4 than in groups 2 and 3 (p>0.05).CONCLUSION:
Administration of apigenin has no significant protective effect on ovarian reserve and tissue damage in ovarian ischemia/reperfusion injury.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Ovarianas
/
Traumatismo por Reperfusão
/
Apigenina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Balkan Med J
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Turquia