Molecular cloning and expression of a glycosyltransferase from Bacillus subtilis for modification of morin and related polyphenols.
Biotechnol Lett
; 39(8): 1229-1235, 2017 Aug.
Article
em En
| MEDLINE
| ID: mdl-28484911
ABSTRACT
OBJECTIVES:
To characterize glycosyltransferases from Bacillus subtilis ATCC 6633 and investigate their substrate specificity towards plant polyphenols.RESULTS:
Among the cloned and expressed six UDP-glycosyltransferases (BsGT1-6), BsGT-1 showed activity with a wide range of polyphenols morin, quercetin, alizarin, rehin, curcumin and aloe emodin. The gene of BsGT-1 has an ORF of 1206 bp encoding 402 amino acids. The recombinant enzyme was purified to homogeneity by Ni-NTA affinity chromatograph, and its biochemical characteristics were identified by HPLC-UV/MS, 1H-NMR and 13C-NMR. BsGT-1 has an MW of approx. 46 kDa as indicated by SDS-PAGE; its activity was optimal at 40 °C and pH 8.5. The Km value of BsGT-1 towards morin was 110 µM.CONCLUSIONS:
BsGT-1 from B. subtilis was cloned. It had high catalytic capabilities towards polyphenols which would make it feasible for the structural modification of polyphenols.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Proteínas de Bactérias
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Flavonoides
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Glicosiltransferases
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Clonagem Molecular
/
Polifenóis
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China