N5 ,N10 -methylenetetrahydromethanopterin reductase from Methanocaldococcus jannaschii also serves as a methylglyoxal reductase.
FEBS Lett
; 591(15): 2269-2278, 2017 08.
Article
em En
| MEDLINE
| ID: mdl-28644554
ABSTRACT
In Methanocaldococcus jannaschii, methylglyoxal (MG) is required for aromatic amino acid biosynthesis. Previously, the reduction of MG to lactaldehyde in Methanocaldococcus jannaschii cell extracts using either NADPH or F420 H2 was demonstrated; however, the enzyme responsible was not identified. Using NADPH as the reductant, the unknown enzyme was purified from cell extracts of Methanocaldococcus jannaschii and determined to be the F420 -dependent N5 ,N10 -methylenetetrahydromethanopterin reductase (Mer). Here, we report that the recombinantly overexpressed Mer is able to use NADPH and MG (KM of 1.6 and 1.0 mm, respectively) to produce lactaldehyde. Additionally, Mer does not catalyze the reduction of MG to lactaldehyde in the presence of reduced Fo, the precursor of F420 .
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases do Álcool
/
Methanocaldococcus
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Oxirredutases atuantes sobre Doadores de Grupo CH-NH
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Estados Unidos