Chemostat Culture for Yeast Physiology and Experimental Evolution.
Cold Spring Harb Protoc
; 2017(7): pdb.top077610, 2017 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-28679718
ABSTRACT
Continuous culture provides many benefits over the classical batch style of growing yeast cells. Steady-state cultures allow for precise control of growth rate and environment. Cultures can be propagated for weeks or months in these controlled environments, which is important for the study of experimental evolution. Despite these advantages, chemostats have not become a highly used system, in large part because of their historical impracticalities, including low throughput, large footprint, systematic complexity, commercial unavailability, high cost, and insufficient protocol availability. However, we have developed methods for building a relatively simple, low-cost, small footprint array of chemostats that can be run in multiples of 32. This "ministat array" can be applied to problems in yeast physiology and experimental evolution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Técnicas Microbiológicas
/
Evolução Molecular
Idioma:
En
Revista:
Cold Spring Harb Protoc
Ano de publicação:
2017
Tipo de documento:
Article