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Escherichia coli DNA ligase B may mitigate damage from oxidative stress.
Bodine, Truston J; Evangelista, Michael A; Chang, Huan Ting; Ayoub, Christopher A; Samuel, Buck S; Sucgang, Richard; Zechiedrich, Lynn.
Afiliação
  • Bodine TJ; Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, United States of America.
  • Evangelista MA; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America.
  • Chang HT; Medical Scientist Training Program, Baylor College of Medicine, Houston, TX, United States of America.
  • Ayoub CA; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America.
  • Samuel BS; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, United States of America.
  • Sucgang R; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America.
  • Zechiedrich L; Department of BioSciences, Rice University, Houston, TX, United States of America.
PLoS One ; 12(7): e0180800, 2017.
Article em En | MEDLINE | ID: mdl-28700629
Escherichia coli encodes two DNA ligases, ligase A, which is essential under normal laboratory growth conditions, and ligase B, which is not. Here we report potential functions of ligase B. We found that across the entire Enterobacteriaceae family, ligase B is highly conserved in both amino acid identity and synteny with genes associated with oxidative stress. Deletion of ligB sensitized E. coli to specific DNA damaging agents and antibiotics resulted in a weak mutator phenotype, and decreased biofilm formation. Overexpression of ligB caused a dramatic extension of lag phase that eventually resumed normal growth. The ligase function of ligase B was not required to mediate the extended lag phase, as overexpression of a ligase-deficient ligB mutant also blocked growth. Overexpression of ligB during logarithmic growth caused an immediate block of cell growth and DNA replication, and death of about half of cells. These data support a potential role for ligase B in the base excision repair pathway or the mismatch repair pathway.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Ligases / Escherichia coli Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Ligases / Escherichia coli Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos