Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HN<sup>dec</sup>-CEST).

Wu, Qinglin; Fenton, Benjamin A; Wojtaszek, Jessica L; Zhou, Pei

Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HN^dec-CEST).

Wu, Qinglin; Fenton, Benjamin A; Wojtaszek, Jessica L; Zhou, Pei.

Afiliação

Wu Q; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA. peizhou@biochem.duke.edu.
Fenton BA; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA. peizhou@biochem.duke.edu.
Wojtaszek JL; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA. peizhou@biochem.duke.edu.
Zhou P; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA. peizhou@biochem.duke.edu.

Chem Commun (Camb) ; 53(61): 8541-8544, 2017 Jul 27.

Article em En | MEDLINE | ID: mdl-28707688

ABSTRACT

ABSTRACT

CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HNdec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HNdec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

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