Molecular pathology of cerebral TNF-α, IL-1ß, iNOS and Nrf2 in forensic autopsy cases with special regard to deaths due to environmental hazards and intoxication.
Forensic Sci Med Pathol
; 13(4): 409-416, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-28776218
ABSTRACT
Deaths involved with environmental hazards and intoxication might present with minimal or nonspecific morphological features, which are insufficient to establish a diagnosis. The present study investigated the postmortem brain mRNA and immunohistochemical expressions of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), inducible nitric oxide synthase (iNOS) and nuclear factor erythroid-2-related factor-2 (Nrf2) in forensic cases. Relative mRNA quantification using Taqman real-time PCR assay demonstrated higher expression of IL-1ß, TNF-α and iNOS, and lower expression of Nrf2 in methamphetamine intoxication and hyperthermia cases, higher expression of iNOS in phenobarbital intoxication cases, and higher expression of Nrf2 in phenobarbital intoxication and hypothermia cases. Immunostaining results showed substantial inter-individual variations in each group, showing no evident differences in distribution or intensity. These findings suggest that different inflammatory and antioxidant responses were involved in deaths from different etiologies, and these markers may be useful for evaluating brain damage and responses.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Fator de Necrose Tumoral alfa
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Óxido Nítrico Sintase Tipo II
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Fator 2 Relacionado a NF-E2
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Interleucina-1beta
Limite:
Adolescent
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Adult
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Aged
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Aged80
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Forensic Sci Med Pathol
Assunto da revista:
JURISPRUDENCIA
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MEDICINA
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PATOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article