Aerobic exercise blocks interleukin-6 levels and germ cell apoptosis in obese rats.

ABSTRACT

To investigate the effect of a high-fat diet and aerobic exercise intervention and its related mechanism on rat germ cell apoptosis. Forty male Sprague-Dawley rats were randomly divided into control group, high-fat diet group, control exercise group and high-fat exercise group. Rats were fed with high-fat diet or were given weight-free swimming. The levels of TG, TC, HDL, LDL and IL-6 in serum of rats were measured. The body weight, body length and inguinal fat weight were measured to calculate the Lee's index and lipid/body weight ratio. The expression of IL-6 mRNA in inguinal fat and IL-6R,Bcl-2 and Bax mRNA in testis was detected by RT-PCR. The morphological structure of testis was observed, and the Johnsen's ten-point score was calculated by HE staining, and the germ cell apoptosis was detected by TUNEL method. We got from the experimental results: a high-fat diet induces obesity and lipid metabolism disorder, alters testis morphological structure and increases germ cell apoptosis in rats. Aerobic exercise improves the lipid metabolism disorder and interferes with germ cell apoptosis by reducing interleukin-6 and interleukin-6 receptor expression.