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Next generation sequencing reveals the antibiotic resistant variants in the genome of Pseudomonas aeruginosa.
Ramanathan, Babu; Jindal, Hassan Mahmood; Le, Cheng Foh; Gudimella, Ranganath; Anwar, Arif; Razali, Rozaimi; Poole-Johnson, Johan; Manikam, Rishya; Sekaran, Shamala Devi.
Afiliação
  • Ramanathan B; Department of Biological Sciences, School of Science and Technology, Sunway University, Kuala Lumpur, Malaysia.
  • Jindal HM; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
  • Le CF; School of Pharmacy, Faculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, Selangor, Malaysia.
  • Gudimella R; Sengenics, High Impact Research (HIR), University of Malaya, Kuala Lumpur, Malaysia.
  • Anwar A; Sengenics, High Impact Research (HIR), University of Malaya, Kuala Lumpur, Malaysia.
  • Razali R; Sengenics, High Impact Research (HIR), University of Malaya, Kuala Lumpur, Malaysia.
  • Poole-Johnson J; Sengenics, High Impact Research (HIR), University of Malaya, Kuala Lumpur, Malaysia.
  • Manikam R; Department of Trauma and Emergency, University Malaya Medical Centre, Kuala Lumpur, Malaysia.
  • Sekaran SD; Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
PLoS One ; 12(8): e0182524, 2017.
Article em En | MEDLINE | ID: mdl-28797043
ABSTRACT
Rapid progress in next generation sequencing and allied computational tools have aided in identification of single nucleotide variants in genomes of several organisms. In the present study, we have investigated single nucleotide polymorphism (SNP) in ten multi-antibiotic resistant Pseudomonas aeruginosa clinical isolates. All the draft genomes were submitted to Rapid Annotations using Subsystems Technology (RAST) web server and the predicted protein sequences were used for comparison. Non-synonymous single nucleotide polymorphism (nsSNP) found in the clinical isolates compared to the reference genome (PAO1), and the comparison of nsSNPs between antibiotic resistant and susceptible clinical isolates revealed insights into the genome variation. These nsSNPs identified in the multi-drug resistant clinical isolates were found to be altering a single amino acid in several antibiotic resistant genes. We found mutations in genes encoding efflux pump systems, cell wall, DNA replication and genes involved in repair mechanism. In addition, nucleotide deletions in the genome and mutations leading to generation of stop codons were also observed in the antibiotic resistant clinical isolates. Next generation sequencing is a powerful tool to compare the whole genomes and analyse the single base pair variations found within the antibiotic resistant genes. We identified specific mutations within antibiotic resistant genes compared to the susceptible strain of the same bacterial species and these findings may provide insights to understand the role of single nucleotide variants in antibiotic resistance.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Farmacorresistência Bacteriana / Antibacterianos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Malásia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Farmacorresistência Bacteriana / Antibacterianos Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Malásia